Evaluating the Role of Biomarkers in Catheter-Related Urogenital Infections

Background: One of the most common infections acquired in the hospital setting is urinary tract infections (UTIs) associated with catheterization. Indwelling urinary catheters are used recurrently in older people.
Objective: Evaluate the diagnostic levels of the cytokines IL-6, TNF-α, IL-8, and calprotectin in the analysis of catheter-related urogenital infection, by using ELISA.
Subjects and Methods: Throughout the period between December 2024 to April 2025, 50 samples were collected serum from patients with diabetes foot surgery with renal failure and 50 serum from healthy person. The participants in this study ranged in age from 51 years and attended the hospitals of the Al-Dwiayniah Health Directorate. Both patient and control three milliliters of venous blood were collected by syringes, then placed in gel tubes and centrifuged at 3000 rpm for 10 minutes, and serum was dispensed into 2 Eppendorf tubes and they were kept at -20°C until testing. ELISA was charity to quantify the absorptions of TNF-α, IL-8, IL-6, and calprotectin.
Results: The results showed the Mean levels of Interleukin-6 were 223.53 ± 21.3 in patients compared with 78.53 ± 11.6. whereas Mean levels of Interleukin-8 were 41.11± 4.63 in patients’ comparison with control 18.90 ± 4.3. Also, the results show the Mean levels of Interleukin TNF-α were 265.83± 15.3 in patients compared with 118.73 ± 11.61. whereas Mean levels of Interleukin calprotectin were 375.04± 38.29 in patients compared with 169.67 ± 21.35. The mean flat was greatly higher than in urinary catheters sick in comparison with well-controlled (P< 0.001).
Conclusion: The results revealed an increased rate of disease in males, 161 (80.5%), in comparison with females, 39 (19.5%). Also, significantly great serum levels of IL-6, TNF-α, IL-8, and calprotectinnin catheterization patients compared to patients who do not have catheter-associated urinary tract infections (CAUTIs).